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KMID : 0384119930130020233
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 2 p.233 ~ p.246
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A Study ofn the Immunologic Surface Markers of Acute Leukemia
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Abstract
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To determine the diagonostic importance of immunophenotypes in acute leukemia, pretreatment peripheral blood or bone marrow blasts from 100 pediatric and adult leukemic patients were analyzed by direct or indirect immunofluorescence method for
reactivity with the monoclonal antibodies to B-cells(CD10, CD19), T-cells(CD2, CD3, CD5, CD7), myeloid antigens(CD13, CD33, CD14, CD41) and nonspecific antigens, HLA-DR. The frequency of three major subgroups of ALL, positive expression rate of
each
monoclonal antibodies(MoAbs) according to leukemic classification with acute mixed lineage leukemia(AMLL) as well as AML-M0 were discussed. Leukemic cells from these patients could be classified as common ALL antigen(DALLA) positive B-ALL(39
cases,
69.6%), CALLA negative B-ALL(10 cases 17.9%) and T-cell All(7 cases, 12.5%). The expression rate of HLA-DR, CD19 and CD10 was 95.9%, 91.7% and 79.6% in B-lineage All, but CD5 and CD2 were expressed in 83.3% and 85.7% of The overall incidence of
AMLL
was 17.0% and all of them were expressed as biphenotypic pattern. As the results, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping, and above data suggest that these monoclonal
antibodies are useful in the characterization of acute leukemia. But to detect more exact AMLL and to establish the relationship between immunologic surface markers and prognosis, follow up study with more additional MoAbs to this study should be
continued.
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KEYWORD
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